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Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae) Anais da ABC (AABC)
Pierre,Patrícia M.O.; Sousa,Saulo M.; Davide,Lisete C.; Machado,Marco A.; Viccini,Lyderson F..
Cytogenetic analyses, of pollen viability, nuclear DNA content and RAPD markers were employed to study three chemotypes of Lippia alba (Mill.) (Verbenaceae) in order to understand the genetic variation among them. Different ploidy levels and mixoploid individuals were observed. This work comprises the first report of different chromosome numbers (cytotypes) in L. alba. The chromosome numbers of La2-carvone and La3-linalool chemotypes suggested that they are polyploids. Flow cytometric analysis showed an increase of nuclear DNA content that was not directly proportional to ploidy level variation. A cluster analysis based on RAPD markers revealed that La3-linalool shares genetic markers with La1-citral and La2-carvone. The analysis showed that the majority...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cheotype; Cytogenetic; Cytotype; DNA content; RAPD; Lippia alba.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000300018
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Relationship between pollen morphology and chromosome numbers in Brazilian species of Lippia L. (Verbenaceae) Anais da ABC (AABC)
Sousa,Saulo M.; Pierre,Patrícia M.O.; Torres,Giovana A.; Davide,Lisete C.; Viccini,Lyderson F..
The pollen morphology and exine structure of 17 species of Lippia L. were investigated in this work using light and scanning electron microscopy. Among the species studied, 14 showed tricolporate pollen grains, two had tri- and tetracolporate pollen grains and a single species exhibited, only tetracolporate pollen. The amb ranged from triangular to square, and the shape varied from oblate-spheroidal to prolate-spheroidal. Three different types of exine ornamentation were observed: psilate, scabrate and perforate. In addition to morphological data, we found positive association between the chromosome numbers and size of pollen grains, and also between the length and width of the colpi. The results indicate that the characteristics of pollen grains in Lippia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome numbers; Lippia; Palynology; Palynomorphology; Pollen; Verbenaceae.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100147
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